The Use Of Positive And Negative Controls To Determine The Macromolecules Present In An Unknown Solution

unravel # 6130, Wednesday, 7-10 PM, B2-151
Lab performed: September 21^st, 2011

Introduction

E precise living thing is dependent on large complex molecules, cognize as macromolecules. The objective of this research laboratory was to correctly identify which macromolecules the unknown solution was comprised of using diverse substances as experimental controls. There are four major types of biological macromolecules - carbohydrates, lipids, proteins, and nucleic acids - made up of elements such as carbon, hydrogen, oxygen, nitrogen, sulfur, and match in various combinations. Macromolecules are essential for survival; they show structural support, a source of stored fuel, the ability to store and obtain genetic information, as well as the ability to further up biochemical reactions; hence their importance in biological systems is notable. (Prakash, 2008).

The control samples have a known outcome; in this lab both incontrovertible and disallow controls were used to determine the identity of the unknown solution. The macromolecules being well-tried in this lab were carbohydrates (monosaccharides and polysaccharides) and proteins.



To identify the figurehead of these macromolecules in a substance, three different foot races were performed. Lugols iodine solution was used for acknowledgment of starch and glycogen - polysaccharides - in the twelve solutions. A positive outcome of the test results in a colour reposition; blue-black in the presence of starch and a red-brown in the presence of glycogen. A negative outcome results in no colour change and all solutions remain a very pale yellow (Pavia, 2005). Benedicts solution was used to identify the presence of reducing sugars; the aldehyde functional group is the part that reacts in the test. A positive indicator of the test is the formation of a benighted precipitate of the blue solution, ranging from yellow-green to red-brown (Hequet and Abidi, 2006). Lastly, the Biuret solution was used to test for the presence of...

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