Purpose- Put desoxyribonucleic acid from one organism into another(prenominal) so the saucy organism can put one across that item protein or protein product it needs.
severalise Ingredients- desoxyribonucleic acid ligase, host cell, obstruction enzymes, vectors
Steps-
i. Plasmids, used as vectors, carry the gene into the host cell
ii. barricade enzymes find the place where the new deoxyribonucleic acid allow for go. It will cut out the old deoxyribonucleic acid and insert the new DNA
iii. DNA ligase is used to secure the new bonds of the new DNA and old DNA
Applications- Used for insulin. Take the DNA of someone who cant make it, put it into an organism that can make it, then condition the protein that is made back in.
PCR
Purpose- to make a massive totality of one gene to be able to study it.
gravestone ingredients- nucleotides, DNA polymerase, thermal cycler, DNA, primers to start DNA replication
Steps-
i. Heat the DNA and add primers. DNA replication occurs.
ii. Primers bond to original DNA
iii. DNA polymerase and nucleotides are added and then cooled to form new DNA
iv. Application- used in forensics to amplify evidence and in gene transfer.
DNA Profiling
Purpose- DNA fingerprinting, compress DNA without looking at the entire genome
Key ingredient- DNA
Steps-
i. Create more(prenominal) DNA using PCR.
ii. The double helix splits into two strands
iii.
Restriction enzymes make DNA into sections
iv. The sections are disjunct using gel electrophoresis (based on size and charge)
v. Creates patterns of stripe and bands according to sequences
vi. Application- forensics and paternity profiling
Human Genome Project
Purpose/application- determine the reparation and structure of all the genes in the human chromosomes, learn more about genetic diseases and how to solve them, evolutionary patterns, who to prone to which diseases
Cloning
Purpose- form new proteins or organisms non-organically
Key ingredients- DNA (from somatic cell), figure out of SCNT, egg cell, host mother
Steps
i. Take out nucleus from...If you indirect request to get a full essay, order it on our website: Ordercustompaper.com
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