Unknown

SIMPLE STAINING:



GRAM STAINING
MATERIALSPROCEDURESHAPECOLOR
1.Slides with love fixed defames.The unknown #14 smear is stained with lechatelierite violet for 20 seconds.

The specimen is shaped like rods.

discovered primarily blue and purple. Therefore, the result is (+).
1.Gram staining fit out and washed nursing bottled.
Washed with water for 2 seconds.
2.Bibulous paper. bourgeon Grams Iodine for one minute.
3.Decolorize with alcoholic drink for 10-20 seconds or until solvents flows colorlessly.
4.Wash with water for 2 seconds.
5.Add safranin solution for one minute.
6.Wash with water for 2 seconds.
7. distinguish dry.

Spore Staining

MATERIALSPROCEDURESHAPECOLOR
1.24-36 hours of nutrient agar of
unknown hum the water in the 5ml beaker. Once water is boiling, place the smear on top of the beaker. Cover the smear with a elflike piece of paper toweling and d saturated with malachite green. steam water boiling water for 5 minutes. Add supererogatory stain if boils off.




Shape like rods.
Observed purple color.We regain the result (+).
2.Electric hot plate and small beaker (25 ml)after the slew has cooled sufficiently crawfish the paper toweling and dust with water for 30 seconds.
3.Spore staining kit consisting of a bottle each of 5% malachite green and saffranine.Counter stain with Safranin for about 20 seconds.
4.Rinse briefly with water to remove Safranin.
5.Blot dry with bibulous paper and examine slide under oil immersion.

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